Evidence of Toxic Factors in the Rumen Liquor of Healthy and Acidotic Buffalo Calves ( Bubalus Bubalis )

Normal and acidotic rumen liquor samples from buffalo calves were subjected to various treatments and injected intraperitoneally to mice. The mortality rate was recorded up to 4B h of post injection. The toxic factors present in the acidotic rumen liquor were heat resistant and were found to be concentrated in the high centrifuged sediment. It was observed that the acidotic rumen liquor was about 1.63 times more toxic than the normal rumen liquor. In the second experiment, the severity of toxicosis and intensity of histopathological alterations were quite apparent between 30 to 45 min. and death often occurred within 3 h of intraperitoneal injections. The toxic factors present in the acidotic rumen liquor might be endotoxic in nature.